Mouse MDSC Flow Cocktail 2

Catalog No. ABIN2669223

Product Details

Reactivity: Mouse

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Isotype Control (IsoC)

Characteristics: This mouse MDSC Flow Cocktail 2 is composed of CD11b, Ly-6C, and Ly-6G antibodies. MDSCs are a heterogenous population of cells that can affect T cell function. Two different subsets of MDSC exist: CD11b+Ly-6G +Ly-6Clow (granulocytic MDSC) and CD11b+Ly-6G-Ly-6Chi (monocytic MDSC). These cells have been identified in a variety of pathological conditions including cancer and autoimmune disease. CD11b: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. Ly-6G: Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. Ly-6C: Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Components: Mouse MDSC Flow Cocktail 2 with Isotype Ctrl, Rat IgG2a/Rat IgG2b/Rat IgG2c, kappa , Clone: M1/70/1A8/HK1.4 25 tests

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.,This reagent is a multicolor cocktail of PE conjugated CD11b (clone M1/70), FITC conjugated Ly-6G (clone 1A8) and APC conjugated Ly-6C (clone HK1.4) at optimal concentration for three-color flow cytometric analysis.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.