2-ThioBarbituric Acid Reactive Substances (TBARS) ELISA Kit
-
- Target
- 2-ThioBarbituric Acid Reactive Substances (TBARS)
- Reactivity
- Chemical
- Detection Method
- Colorimetric
- Application
- ELISA
- Sample Type
- Serum, Plasma, Urine
- Sensitivity
- 0.5 μM
- Characteristics
- 2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This TBARS Cuvette Assay Kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 0.5 h
- Plate
- Pre-coated
- Protocol
- This TBARS Cuvette Assay Kit is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25 °C. One molecule of MDA reacts with 2 Molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532 nm,
- Restrictions
- For Research Use only
-
- Storage
- 4 °C
-
- Target
- 2-ThioBarbituric Acid Reactive Substances (TBARS)
-