Ubiquinone / Coenzyme Q10 HPLC Assay
HPLC
Reactivity: Chemical
Blood (EDTA), Plasma, Serum
-
- Target See all Coenzyme Q10 (CoQ10) products
- Coenzyme Q10 (CoQ10)
-
Reactivity
- Chemical
- Application
- High Pressure Liquid Chromatography (HPLC)
- Purpose
- Ubiquinone is a coenzyme of every cell in the body. Ubiquinone is made up by a chinonic ring and an isoprenic side chain. In humans ubiquinone can be synthesized and taken up by nutrition.
- Sample Type
- Serum, Plasma, Blood (EDTA)
- Characteristics
- Ubiquinone is a coenzyme of every cell in the body. Ubiquinone is made up by a chinonic ring and an isoprenic side chain. In humans ubiquinone can be synthesized and taken up by nutrition.
-
Coenzyme Q10 (CoQ10) ELISA Kit
CoQ10 Reactivity: Human Colorimetric Competition ELISA 3.12 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 200 μL
- Assay Time
- 0.5 h
- Protocol
- For the determination of ubiquinone (coenzyme Q10) a precipitation step which removes high molecular substances is performed first. The internal standard reduces the variation in the sample treatment. After centrifugation the supernatant is mixed with an extraction solution and transferred to the organic phase. The organic solvent is evaporated and the sample is re-suspended in ethanol p.A. and injected into the HPLC system. The isocratic separation via HPLC at 30 °C using a "reversed phase" column needs 15 minutes. The chromatograms are recorded by a UV-detector. The quantification is performed by the delivered calibrator and the concentration is calculated by the "internal standard method" via integration of the peak areas resp. peak heights.
- Restrictions
- For Research Use only
-
- Storage
- 4 °C
-
- Target
- Coenzyme Q10 (CoQ10)
- Alternative Name
- Ubiquinone / Coenzyme Q10 (CoQ10 Products)
-
You are here:
