Pepsinogen A ELISA Kit

Catalog No. ABIN2648811

Product Details Pepsinogen A ELISA Kit

Target: Pepsinogen A

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum

Sensitivity: 0.1 ng/ml

Characteristics: This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative determination of human pepsinogen I levels in serum. Human Pepsinogen ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 25 μL

Assay Time: 1.5 h

Protocol: Human Pepsinogen I ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human pepsinogen I level in serum sample. The assay utilizes the two-site "sandwich" technique with two selected monoclonal antibodies that bind to different epitopes of human pepsinogen I without any cross-reaction to human pepsinogen II. Assay standards, controls and patient serum samples containing human pepsinogen I is added directly to microtiter wells of microplate that was coated with a streptavidin. Simultaneously, a biotinylated antibody and a horseradish peroxidase conjugated antibody are added to each well. After the first incubation period, on the wall of microtiter well captures the biotinylated antibody as well as an immunocomplex in the form of "streptavidin - biotin-antibody - pepsinogen I- HRP-antibody". Unbound proteins as well as unbound HRP conjugated antibody in each microtiter well are removed in the subsequent washing step. The well is incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to the pepsinogen I on the wall of the microtiter well is directly proportional to the amount of pepsinogen I in the sample. A standard curve is generated by plotting the absorbance versus the respective human pepsinogen I concentration for each standard on Point-to-Point, CubicSpline or 4-Parameter plot. The concentration of human pepsinogen I in test samples is determined directly from this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Pepsinogen A

Target: Pepsinogen A

Alternative Name: Pepsinogen I (Pepsinogen A Products)

Synonyms: PGA3 ELISA Kit, PGA4 ELISA Kit, PGA5 ELISA Kit, PGNA ELISA Kit, CPGA ELISA Kit, pga ELISA Kit, pepsinogen 5, group I (pepsinogen A) ELISA Kit, pepsinogen A ELISA Kit, PGA5 ELISA Kit, PY01268 ELISA Kit, CC1G_11543 ELISA Kit, PG ELISA Kit, PGA ELISA Kit