IL-32 alpha ELISA Kit

Catalog No. ABIN2648765

Product Details IL-32 alpha ELISA Kit

Target: IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Purpose: Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 32 Alpha (IL-32α) concentrations in cell culture supernates, serum, and plasma.

Sample Type: Serum, Plasma, Cell Culture Cells

Analytical Method: Quantitative

Sensitivity: 7 pg/ml

Characteristics: Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Human Interleukin 32 Alpha (IL-32α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-32α ? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-32α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-32α is added to the wells and binds to the combination of capture antibody- IL-32α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-32α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-32α standard dilutions and IL-32α sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-32 alpha

Target: IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))

Alternative Name: IL-32a (IL32A Products)