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IL-9 ELISA Kit

IL9 Reactivity: Human Colorimetric Cell Culture Cells, Plasma, Serum
Catalog No. ABIN2648706
  • Target See all IL-9 (IL9) ELISA Kits
    IL-9 (IL9) (Interleukin 9 (IL9))
    Reactivity
    • 7
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Application
    ELISA
    Purpose
    Human Interleukin 9 (IL-9) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 9 (IL-9) concentrations in cell culture supernates, serum, and plasma.
    Sample Type
    Serum, Plasma, Cell Culture Cells
    Analytical Method
    Quantitative
    Sensitivity
    2 pg/ml
    Characteristics
    Human Interleukin 9 (IL-9) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Sample Volume
    100 μL
    Assay Time
    3.5 h
    Plate
    Pre-coated
    Protocol
    Human Interleukin 9 (IL-9) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-9 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-9 is added to the wells and binds to the combination of capture antibody- IL-9 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-9 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-9 standard dilutions and IL-9 sample concentration determined.
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Target See all IL-9 (IL9) ELISA Kits
    IL-9 (IL9) (Interleukin 9 (IL9))
    Alternative Name
    IL-9 (IL9 Products)
    Synonyms
    IL9 ELISA Kit, IL-9 ELISA Kit, Il-9 ELISA Kit, P40 ELISA Kit, HP40 ELISA Kit, interleukin 9 ELISA Kit, IL9 ELISA Kit, il9 ELISA Kit, Il9 ELISA Kit
    Pathways
    JAK-STAT Signaling
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