ENAH ELISA Kit
ENAH
Reactivity: Human
Colorimetric
Plasma, Serum
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- Target See all ENAH products
- ENAH (Enabled Homolog (ENAH))
- Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Sample Type
- Serum, Plasma
- Sensitivity
- Cut Off Control
- Characteristics
- Systemic autoimmune diseases such as systemic lupus erythematosus, scleroderma, rheumatoid arthritis, Sjögren's syndrome, dermatomyositis, mixed connective tissue disease are characterized by the appearance of a variety of autoantibodies directed against components of the cell nucleus.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 2 h
- Plate
- Pre-coated
- Protocol
- The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3',5,5'-tetramethyl-benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
-
- Target See all ENAH products
- ENAH (Enabled Homolog (ENAH))
- Alternative Name
- ENA (Extractable Nuclear Antigen) (ENAH Products)
- Synonyms
- ENA ELISA Kit, MENA ELISA Kit, NDPP1 ELISA Kit, AI464316 ELISA Kit, AW045240 ELISA Kit, Mena ELISA Kit, Ndpp1 ELISA Kit, WBP8 ELISA Kit, id:ibd2798 ELISA Kit, si:ch211-215p14.1 ELISA Kit, zgc:103638 ELISA Kit, ENAH, actin regulator ELISA Kit, enabled homolog (Drosophila) ELISA Kit, Enah ELISA Kit, ENAH ELISA Kit, enah ELISA Kit
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