G-CSF ELISA Kit
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- Target See all G-CSF (CSF3) ELISA Kits
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Purpose
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Colony Stimulating Factor (G-CSF) concentrations in cell culture supernates, serum, and plasma.
- Sample Type
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- Sensitivity
- 4 pg/ml
- Characteristics
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 3.5 h
- Plate
- Pre-coated
- Protocol
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for G-CSF is added to the wells and binds to the combination of capture antibody- G-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of G-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven G-CSF standard dilutions and G-CSF sample concentration determined.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all G-CSF (CSF3) ELISA Kits
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- Alternative Name
- G-CSF (CSF3 Products)
- Synonyms
- CSF3 ELISA Kit, G-CSF ELISA Kit, gcsf ELISA Kit, Csfg ELISA Kit, MGI-IG ELISA Kit, C17orf33 ELISA Kit, CSF3OS ELISA Kit, GCSF ELISA Kit, Gcsf ELISA Kit, colony stimulating factor 3 ELISA Kit, colony stimulating factor 3 (granulocyte) a ELISA Kit, colony stimulating factor 3 (granulocyte) ELISA Kit, CSF3 ELISA Kit, csf3a ELISA Kit, Csf3 ELISA Kit
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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