Biotin ELISA Kit

Catalog No. ABIN2648517

Product Details Biotin ELISA Kit

Target: Biotin

Reactivity: Chemical

Detection Method: Colorimetric

Application: ELISA

Sample Type: Food

Sensitivity: 0.5 ng/mL

Characteristics: Biotin serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysin residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1.5 h

Plate: Pre-coated

Protocol: Biotin (Vitamin H) quantitative test is based on the principle of the enzyme linked immu-nosorbent assay. Avidin, which shows a high affinity to biotin, is bound on the surface of a microtiter plate. Biotin containing samples or standards and a biotin-alkaline phosphatase conjugate are given into the wells of the microtiter plate. Enzyme labelled and free biotin compete for the binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove un-bound material. A substrate solution is added and incubated for 30 minutes, resulting in the develop-ment of a yellow colour. The colour development is inhibited by the addition of a stop solution. The yellow colour is measured photometrically at 405 nm. The concentra-tion of biotin is indirectly proportional to the colour inten-sity of the Biotin ELISA Assay test sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical