TJP1 ELISA Kit
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- TJP1 (Tight Junction Protein 1 (TJP1))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.15-10 ng/mL
- Minimum Detection Limit
- 0.15 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.051 ng/mL
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- Plate
- Pre-coated
- Protocol
- Tight Junction Protein 1 (TJP1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tight Junction Protein 1 (TJP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tight Junction Protein 1 (TJP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tight Junction Protein 1 (TJP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Tight Junction Protein 1 (TJP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all TJP1 ELISA Kits
- TJP1 (Tight Junction Protein 1 (TJP1))
- Abstract
- TJP1 Products
- Synonyms
- ZO-1 ELISA Kit, ZO1 ELISA Kit, zo1 ELISA Kit, tight junction protein 1 ELISA Kit, tight junction protein ZO-1 ELISA Kit, TJP1 ELISA Kit, Tjp1 ELISA Kit, LOC396567 ELISA Kit
- Pathways
- Carbohydrate Homeostasis, Cell-Cell Junction Organization
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