CCL28 ELISA Kit

Catalog No. ABIN2043995

Product Details CCL28 ELISA Kit

Target: CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Mucosae Associated Epithelia Chemokine (MEC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucosae Associated Epithelia Chemokine (MEC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucosae Associated Epithelia Chemokine (MEC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucosae Associated Epithelia Chemokine (MEC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mucosae Associated Epithelia Chemokine (MEC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CCL28

Target: CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))

Alternative Name: Mucosae Associated Epithelia Chemokine (MEC) (CCL28 Products)