IL2RG ELISA Kit

Catalog No. ABIN2043807

Product Details IL2RG ELISA Kit

Target: IL2RG (Interleukin 2 Receptor, gamma (IL2RG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Interleukin 2 Receptor Gamma (IL2Rg) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Gamma (IL2Rg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 Receptor Gamma (IL2Rg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 Receptor Gamma (IL2Rg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 2 Receptor Gamma (IL2Rg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL2RG

Target: IL2RG (Interleukin 2 Receptor, gamma (IL2RG))

Alternative Name: Interleukin 2 Receptor gamma (IL2Rg) (IL2RG Products)

Pathways: JAK-STAT Signaling, Growth Factor Binding