Integrin beta 5 ELISA Kit
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- Target See all Integrin beta 5 (ITGB5) ELISA Kits
- Integrin beta 5 (ITGB5)
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.15-10 ng/mL
- Minimum Detection Limit
- 0.15 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.065 ng/mL
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- Plate
- Pre-coated
- Protocol
- Integrin Beta 5 (ITGb5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 5 (ITGb5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 5 (ITGb5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 5 (ITGb5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Integrin Beta 5 (ITGb5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all Integrin beta 5 (ITGB5) ELISA Kits
- Integrin beta 5 (ITGB5)
- Abstract
- ITGB5 Products
- Synonyms
- ITGB5 ELISA Kit, AA475909 ELISA Kit, AI874634 ELISA Kit, ESTM23 ELISA Kit, [b]-5 ELISA Kit, [b]5 ELISA Kit, [b]5A ELISA Kit, [b]5B ELISA Kit, beta-5 ELISA Kit, beta5 ELISA Kit, RGD1563276 ELISA Kit, integrin, beta 5 ELISA Kit, integrin beta 5 ELISA Kit, integrin subunit beta 5 ELISA Kit, ITGB5 ELISA Kit, Itgb5 ELISA Kit
- Pathways
- Integrin Complex
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