CD235a/GYPA ELISA Kit
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- Target See all CD235a/GYPA (GYPA) ELISA Kits
- CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.31-20 ng/mL
- Minimum Detection Limit
- 0.31 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.134 ng/mL
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Glycophorin A (GYPA) ELISA Kit
GYPA Reactivity: Mouse Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Glycophorin A (GYPA) ELISA KitGYPA Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
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- Plate
- Pre-coated
- Protocol
- Glycophorin A (GYPA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycophorin A (GYPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycophorin A (GYPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycophorin A (GYPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glycophorin A (GYPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all CD235a/GYPA (GYPA) ELISA Kits
- CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
- Abstract
- GYPA Products
- Pathways
- Maintenance of Protein Location
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