FGF21 ELISA Kit
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- Target See all FGF21 ELISA Kits
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 7.81-500 pg/mL
- Minimum Detection Limit
- 7.81 pg/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 3.2 pg/mL
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- Discover our best selling FGF21 ELISA Kit
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- Discover our top product FGF21 ELISA Kit
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- Plate
- Pre-coated
- Protocol
- Fibroblast Growth Factor 21 (FGF21) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 21 (FGF21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 21 (FGF21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 21 (FGF21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fibroblast Growth Factor 21 (FGF21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all FGF21 ELISA Kits
- FGF21 (Fibroblast Growth Factor 21 (FGF21))
- Abstract
- FGF21 Products
- Synonyms
- FGF21 ELISA Kit, fibroblast growth factor 21 ELISA Kit, FGF21 ELISA Kit, fgf21 ELISA Kit, Fgf21 ELISA Kit
- Pathways
- RTK Signaling
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