DKK4 ELISA Kit
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- DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.15-10 ng/mL
- Minimum Detection Limit
- 0.15 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.051 ng/mL
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- Plate
- Pre-coated
- Protocol
- Dickkopf Related Protein 4 (DKK4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 4 (DKK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 4 (DKK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 4 (DKK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dickkopf Related Protein 4 (DKK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all DKK4 ELISA Kits
- DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))
- Alternative Name
- Dickkopf Related Protein 4 (DKK4) (DKK4 Products)
- Synonyms
- DKK-4 ELISA Kit, Dkk-4 ELISA Kit, RGD1563172 ELISA Kit, dickkopf WNT signaling pathway inhibitor 4 ELISA Kit, DKK4 ELISA Kit, Dkk4 ELISA Kit
- Pathways
- WNT Signaling
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