This Colorimetric ELISA kit is designed for the quantitative measurement of Human CA 19-5.
Catalog No. ABIN2043065
Quick Overview for CA 19-5 ELISA Kit (ABIN2043065)
Target
CA 19-5
(Carbohydrate Antigen 19-5 (CA 19-5))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Competition ELISA
Detection Range
2.47-200 ng/mL
Application
ELISA
Minimum Detection Limit
2.47 ng/mL
Brand
BioAssay™
Sensitivity
< 0.86 ng/mL
Plate
Pre-coated
Protocol
Carbohydrate Antigen 19-5 (CA19-5) BioAssay-ELISA Kit (Human) employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Carbohydrate Antigen 19-5 (CA19-5) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Carbohydrate Antigen 19-5 (CA19-5) and unlabeled Carbohydrate Antigen 19-5 (CA19-5) (Standards or samples) with the pre-coated antibody specific for Carbohydrate Antigen 19-5 (CA19-5). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Carbohydrate Antigen 19-5 (CA19-5) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carbohydrate Antigen 19-5 (CA19-5) in the sample.