CD80 ELISA Kit
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- Target See all CD80 ELISA Kits
- CD80
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 62.5-4000 pg/mL
- Minimum Detection Limit
- 62.5 pg/mL
- Application
- ELISA
- Brand
- BioAssay™
- Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- < 10 pg/mL
- Components
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96-well plate pre-coated with anti-B7-1/CD80, Mouse
Lyophilized recombinant B7-1/CD80, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL - Featured
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- Plate
- Pre-coated
- Protocol
- Sandwich ELISA quantitative detection kit for Mouse B7-1/CD80 in cell culture supernates and serum.The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1 D37-K245 which is composed of two single chains acids with the dipolymer.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all CD80 ELISA Kits
- CD80
- Alternative Name
- B7-1/CD80 (CD80 Products)
- Synonyms
- B7 ELISA Kit, B7-1 ELISA Kit, B7.1 ELISA Kit, BB1 ELISA Kit, CD28LG ELISA Kit, CD28LG1 ELISA Kit, LAB7 ELISA Kit, B71 ELISA Kit, Cd28l ELISA Kit, Ly-53 ELISA Kit, Ly53 ELISA Kit, MIC17 ELISA Kit, TSA1 ELISA Kit, CD80 molecule ELISA Kit, Cd80 molecule ELISA Kit, CD80 antigen ELISA Kit, CD80 ELISA Kit, Cd80 ELISA Kit
- Gene ID
- 12519
- UniProt
- Q00609
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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