TIMP2 ELISA Kit

Catalog No. ABIN1889442

Product Details TIMP2 ELISA Kit

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Binding Specificity: AA 27-220

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat TIMP-2

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: C27-P220

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: C27-P220

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

Plate: Pre-coated

Protocol: rat TIMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-2 has been precoated onto 96-well plates. Standards(NSO, C27-P220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TIMP-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL rat TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat TIMP-2 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.17, Standard deviation: 0.051, CV(%): 4.4
• Sample 2: n=16, Mean(ng/ml): 2.9, Standard deviation: 0.168, CV(%): 5.8
• Sample 3: n=16, Mean(ng/ml): 5.85, Standard deviation: 0.368, CV(%): 6.3,
• Sample 1: n=24, Mean(ng/ml): 1.21, Standard deviation: 0.091, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 3.55, Standard deviation: 0.256, CV(%): 7.2
• Sample 3: n=24, Mean(ng/ml): 6.38, Standard deviation: 0.408, CV(%): 6.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for TIMP2 ELISA Kit (ABIN1889442)

Zhang, Shi, Zou, Chen, Tang, Ye, Liu: "High glucose stimulates cell proliferation and Collagen IV production in rat mesangial cells through inhibiting AMPK-KATP signaling." in: International urology and nephrology, Vol. 49, Issue 11, pp. 2079-2086, (2018) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Cavdar, Ozbal, Celik, Ergur, Guneli, Ural, Camsari, Guner: "The effects of alpha-lipoic acid on MMP-2 and MMP-9 activities in a rat renal ischemia and re-perfusion model." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 4, pp. 304-14, (2014) (PubMed).

Xu, Ling, Zhu, Fan, Zhang: "The effect of 2,3,4',5-tetrahydroxystilbene-2-0-?-D glucoside on neointima formation in a rat artery balloon injury model and its possible mechanisms." in: European journal of pharmacology, Vol. 698, Issue 1-3, pp. 370-8, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Yu, Zhu, Mi, Chen, Pan, Wei: "Anti-angiogenic genistein inhibits VEGF-induced endothelial cell activation by decreasing PTK activity and MAPK activation." in: Medical oncology (Northwood, London, England), Vol. 29, Issue 1, pp. 349-57, (2012) (PubMed).

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2 (TIMP2 Products)

Synonyms: CSC-21K ELISA Kit, DDC8 ELISA Kit, timp2l ELISA Kit, zgc:65967 ELISA Kit, zgc:85799 ELISA Kit, D11Bwg1104e ELISA Kit, Timp-2 ELISA Kit, TIMP-2 ELISA Kit, etID32613.12 ELISA Kit, fa96h09 ELISA Kit, timp2 ELISA Kit, wu:fa96h09 ELISA Kit, zgc:136726 ELISA Kit, TIMP metallopeptidase inhibitor 2 ELISA Kit, TIMP metallopeptidase inhibitor 2b ELISA Kit, tissue inhibitor of metalloproteinase 2 ELISA Kit, TIMP metallopeptidase inhibitor 2a ELISA Kit, TIMP metallopeptidase inhibitor 2 L homeolog ELISA Kit, TIMP2 ELISA Kit, timp2b ELISA Kit, Timp2 ELISA Kit, timp2a ELISA Kit, timp2.L ELISA Kit

Background:

Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Background: TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Synonyms: Metalloproteinase inhibitor 2,Tissue inhibitor of metalloproteinases 2,TIMP-2,Timp2,Timp-2,

Full Gene Name: Metalloproteinase inhibitor 2

Cellular Localisation: Secreted.

Gene ID: 29543

UniProt: P30121

Pathways: cAMP Metabolic Process