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DLL1 ELISA Kit

DLL1 Reactivity: Human AA 22-540 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum, Urine
Catalog No. ABIN1889418
  • Target See all DLL1 ELISA Kits
    DLL1 (delta-Like 1 (DLL1))
    Binding Specificity
    AA 22-540
    Reactivity
    • 6
    • 3
    • 3
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78-5000 pg/mL
    Minimum Detection Limit
    78 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DLL1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine, Milk
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: S22-G540
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: S22-G540
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 DSL domain.

    Tissue Specificity: Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

    Plate
    Pre-coated
    Protocol
    human DLL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DLL1 has been precoated onto 96-well plates. Standards(NSO, S22-G540) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DLL1 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human DLL1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human DLL1 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 713, Standard deviation: 24.9, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 1579, Standard deviation: 64.74, CV(%): 4.1
    • Sample 3: n=16, Mean(pg/ml): 3618, Standard deviation: 137.5, CV(%): 3.8,
    • Sample 1: n=24, Mean(pg/ml): 674, Standard deviation: 49.8, CV(%): 7.4
    • Sample 2: n=24, Mean(pg/ml): 1486, Standard deviation: 101, CV(%): 6.8
    • Sample 3: n=24, Mean(pg/ml): 3542, Standard deviation: 269.2, CV(%): 7.6
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all DLL1 ELISA Kits
    DLL1 (delta-Like 1 (DLL1))
    Alternative Name
    DLL1 (DLL1 Products)
    Synonyms
    X-delta-1 ELISA Kit, XDelta1 ELISA Kit, Xdelta-1 ELISA Kit, delta ELISA Kit, delta-1 ELISA Kit, delta1 ELISA Kit, x-delta ELISA Kit, DELTA1 ELISA Kit, DL1 ELISA Kit, Delta ELISA Kit, DLK ELISA Kit, DLK-1 ELISA Kit, Delta1 ELISA Kit, FA1 ELISA Kit, PREF1 ELISA Kit, Pref-1 ELISA Kit, ZOG ELISA Kit, pG2 ELISA Kit, DLL1 ELISA Kit, AW742678 ELISA Kit, DlkI ELISA Kit, Ly107 ELISA Kit, Peg9 ELISA Kit, SCP1 ELISA Kit, pref-1 ELISA Kit, delta-like 1 ELISA Kit, delta like canonical Notch ligand 1 ELISA Kit, delta like non-canonical Notch ligand 1 ELISA Kit, delta-like 1 L homeolog ELISA Kit, delta-like 1 (Drosophila) ELISA Kit, delta-like 1 homolog (Drosophila) ELISA Kit, dll1 ELISA Kit, DLL1 ELISA Kit, DLK1 ELISA Kit, Dll1 ELISA Kit, dll1.L ELISA Kit, Dlk1 ELISA Kit
    Background

    Protein Function: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. .

    Background: DLL1(DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

    Synonyms: Delta-like protein 1,Drosophila Delta homolog 1,Delta1,H-Delta-1,DLL1,UNQ146/PRO172,

    Full Gene Name: Delta-like protein 1

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gene ID
    28514
    UniProt
    O00548
    Pathways
    Notch Signaling, Stem Cell Maintenance
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