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PRSS8 ELISA Kit

PRSS8 Reactivity: Mouse AA 30-289 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Catalog No. ABIN1889415
  • Target See all PRSS8 ELISA Kits
    PRSS8 (Protease, serine, 8 (PRSS8))
    Binding Specificity
    AA 30-289
    Reactivity
    • 9
    • 5
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.78-50 ng/mL
    Minimum Detection Limit
    0.78 ng/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Prostasin
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: A30-Q289
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <50pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: A30-Q289
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Belongs to the peptidase S1 family.

    Plate
    Pre-coated
    Protocol
    mouse Prostasin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Prostasin has been precoated onto 96-well plates. Standards(NSO, A30-Q289) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prostasin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Prostasin amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Prostasin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Prostasin standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.45, Standard deviation: 0.08, CV(%): 5.6
    • Sample 2: n=16, Mean(ng/ml): 25.2, Standard deviation: 1.06, CV(%): 4.2
    • Sample 3: n=16, Mean(ng/ml): 38.1, Standard deviation: 2.02, CV(%): 5.3,
    • Sample 1: n=24, Mean(ng/ml): 2.64, Standard deviation: 0.19, CV(%): 7.2
    • Sample 2: n=24, Mean(ng/ml): 18.5, Standard deviation: 1.18, CV(%): 6.4
    • Sample 3: n=24, Mean(ng/ml): 40.7, Standard deviation: 3.5, CV(%): 8.6
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all PRSS8 ELISA Kits
    PRSS8 (Protease, serine, 8 (PRSS8))
    Alternative Name
    PRSS8 (PRSS8 Products)
    Synonyms
    CAP1 ELISA Kit, PROSTASIN ELISA Kit, prostasin ELISA Kit, 2410039E18Rik ELISA Kit, AI313909 ELISA Kit, C79772 ELISA Kit, fr ELISA Kit, mCAP1 ELISA Kit, protease, serine 8 ELISA Kit, protease, serine, 8 ELISA Kit, protease, serine 8 (prostasin) ELISA Kit, PRSS8 ELISA Kit, Prss8 ELISA Kit
    Background

    Protein Function: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates (By similarity). Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). .

    Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60 % to 80 % increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

    Synonyms: Prostasin,3.4.21.-,Channel-activating protease 1,CAP1,Serine protease 8,Prostasin light chain,Prostasin heavy chain,Prss8,Cap1,

    Full Gene Name: Prostasin

    Cellular Localisation: Cell membrane, Single-pass membrane protein.
    Gene ID
    76560
    UniProt
    Q9ESD1
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