PRSS8 ELISA Kit
-
- Target See all PRSS8 ELISA Kits
- PRSS8 (Protease, serine, 8 (PRSS8))
- Binding Specificity
- AA 30-289
-
Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.78-50 ng/mL
- Minimum Detection Limit
- 0.78 ng/mL
- Application
- ELISA
- Purpose
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Prostasin
- Brand
- PicoKine™
- Sample Type
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- Specificity
-
Expression system for standard: NSO
Immunogen sequence: A30-Q289 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Sensitivity
- <50pg/mL
- Material not included
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- Immunogen
-
Expression system for standard: NSO
Immunogen sequence: A30-Q289 - Featured
- Discover our best selling PRSS8 ELISA Kit
- Top Product
- Discover our top product PRSS8 ELISA Kit
-
-
- Application Notes
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- Comment
-
Sequence similarities: Belongs to the peptidase S1 family.
- Plate
- Pre-coated
- Protocol
- mouse Prostasin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Prostasin has been precoated onto 96-well plates. Standards(NSO, A30-Q289) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prostasin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Prostasin amount of sample captured in plate.
- Assay Procedure
-
Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Prostasin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Prostasin standard solution and each sample is measured in duplicate.
- Assay Precision
-
- Sample 1: n=16, Mean(ng/ml): 1.45, Standard deviation: 0.08, CV(%): 5.6
- Sample 2: n=16, Mean(ng/ml): 25.2, Standard deviation: 1.06, CV(%): 4.2
- Sample 3: n=16, Mean(ng/ml): 38.1, Standard deviation: 2.02, CV(%): 5.3,
- Sample 1: n=24, Mean(ng/ml): 2.64, Standard deviation: 0.19, CV(%): 7.2
- Sample 2: n=24, Mean(ng/ml): 18.5, Standard deviation: 1.18, CV(%): 6.4
- Sample 3: n=24, Mean(ng/ml): 40.7, Standard deviation: 3.5, CV(%): 8.6
- Restrictions
- For Research Use only
-
- Handling Advice
- Avoid multiple freeze-thaw cycles.
- Storage
- -20 °C,4 °C
- Storage Comment
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- Expiry Date
- 12 months
-
- Target See all PRSS8 ELISA Kits
- PRSS8 (Protease, serine, 8 (PRSS8))
- Alternative Name
- PRSS8 (PRSS8 Products)
- Synonyms
- CAP1 ELISA Kit, PROSTASIN ELISA Kit, prostasin ELISA Kit, 2410039E18Rik ELISA Kit, AI313909 ELISA Kit, C79772 ELISA Kit, fr ELISA Kit, mCAP1 ELISA Kit, protease, serine 8 ELISA Kit, protease, serine, 8 ELISA Kit, protease, serine 8 (prostasin) ELISA Kit, PRSS8 ELISA Kit, Prss8 ELISA Kit
- Background
-
Protein Function: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates (By similarity). Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). .
Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60 % to 80 % increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.
Synonyms: Prostasin,3.4.21.-,Channel-activating protease 1,CAP1,Serine protease 8,Prostasin light chain,Prostasin heavy chain,Prss8,Cap1,
Full Gene Name: Prostasin
Cellular Localisation: Cell membrane, Single-pass membrane protein. - Gene ID
- 76560
- UniProt
- Q9ESD1
-