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CD320 ELISA Kit

CD320 Reactivity: Human AA 36-229 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Catalog No. ABIN1889407
  • Target See all CD320 ELISA Kits
    CD320 (CD320 Molecule (CD320))
    Binding Specificity
    AA 36-229
    Reactivity
    • 5
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10.000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD320/8D6A
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: S36-Y229
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: S36-Y229
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Expressed abundantly on follicular dendritic cells (FDCs). .

    Plate
    Pre-coated
    Protocol
    human CD320 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD320 has been precoated onto 96-well plates. Standards(NSO, S36-Y229) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD320 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD320 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD320 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD320 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.1, Standard deviation: 0.042, CV(%): 3.8
    • Sample 2: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.14, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 6.3, Standard deviation: 0.31, CV(%): 4.9,
    • Sample 1: n=24, Mean(ng/ml): 1.3, Standard deviation: 0.08, CV(%): 6.2
    • Sample 2: n=24, Mean(ng/ml): 3.4, Standard deviation: 0.194, CV(%): 5.7
    • Sample 3: n=24, Mean(ng/ml): 5.9, Standard deviation: 0.313, CV(%): 5.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CD320 ELISA Kits
    CD320 (CD320 Molecule (CD320))
    Alternative Name
    CD320 (CD320 Products)
    Synonyms
    8D6 ELISA Kit, 8D6A ELISA Kit, TCBLR ELISA Kit, 425O18-1 ELISA Kit, D17Ertd716e ELISA Kit, NG29 ELISA Kit, VLDL ELISA Kit, RGD1305860 ELISA Kit, TCblR ELISA Kit, CD320 molecule ELISA Kit, CD320 antigen ELISA Kit, CD320 ELISA Kit, Cd320 ELISA Kit
    Background

    Protein Function: Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. .

    Background: CD320(cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin(vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.

    Synonyms: CD320 antigen,8D6 antigen,FDC-signaling molecule 8D6,FDC-SM-8D6,Transcobalamin receptor,TCblR,CD320,CD320,8D6A,UNQ198/PRO224,

    Full Gene Name: CD320 antigen

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gene ID
    51293
    UniProt
    Q9NPF0
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