Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Renin ELISA Kit

REN Reactivity: Mouse AA 72-402 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889403
  • Target See all Renin (REN) ELISA Kits
    Renin (REN)
    Binding Specificity
    AA 72-402
    Reactivity
    • 10
    • 9
    • 8
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Renin-1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: S72-R402
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: S72-R402
    Featured
    Discover our best selling REN ELISA Kit
    Top Product
    Discover our top product REN ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Kidney.

    Plate
    Pre-coated
    Protocol
    mouse Renin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Renin-1 has been precoated onto 96-well plates. Standards(NSO, S72-R402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Renin-1 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Renin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Renin-1 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 890, Standard deviation: 49.84, CV(%): 5.6
    • Sample 2: n=16, Mean(pg/ml): 1137, Standard deviation: 48.9, CV(%): 4.3
    • Sample 3: n=16, Mean(pg/ml): 2932, Standard deviation: 161.26, CV(%): 5.5,
    • Sample 1: n=24, Mean(pg/ml): 927, Standard deviation: 67.7, CV(%): 7.3
    • Sample 2: n=24, Mean(pg/ml): 2274, Standard deviation: 129.6, CV(%): 5.7
    • Sample 3: n=24, Mean(pg/ml): 3045, Standard deviation: 194.88, CV(%): 6.4
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Wang, Ren: "Preparation and characterization of polyclonal antibody against severe acute respiratory syndrome-associated coronavirus spike protein." in: Hybridoma (2005), Vol. 29, Issue 6, pp. 511-6, (2010) (PubMed).

  • Target See all Renin (REN) ELISA Kits
    Renin (REN)
    Alternative Name
    Renin-1 (REN Products)
    Synonyms
    HNFJ2 ELISA Kit, RATRENAA ELISA Kit, RENAA ELISA Kit, Ren1 ELISA Kit, D19352 ELISA Kit, Ren ELISA Kit, Ren-1 ELISA Kit, Ren-A ELISA Kit, Ren1c ELISA Kit, Ren1d ELISA Kit, Rn-1 ELISA Kit, Rnr ELISA Kit, renin ELISA Kit, renin 1 structural ELISA Kit, REN ELISA Kit, Ren ELISA Kit, Ren1 ELISA Kit, ren ELISA Kit
    Background

    Protein Function: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

    Background: Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.

    Synonyms: Renin-1,3.4.23.15,Angiotensinogenase,Kidney renin,Ren1,Ren, Ren-1,

    Full Gene Name: Renin-1

    Cellular Localisation: Secreted . Membrane . Associated to membranes via binding to ATP6AP2..
    Gene ID
    19701
    UniProt
    P06281
    Pathways
    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
You are here:
Support