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CCL9 ELISA Kit

Ccl9 Reactivity: Mouse AA 22-122 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889389
  • Target See all CCL9 (Ccl9) ELISA Kits
    CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
    Binding Specificity
    AA 22-122
    Reactivity
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    7.8-500 pg/mL
    Minimum Detection Limit
    7.8 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL9
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: Q22-Q122
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <1pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: Q22-Q122
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.

    Plate
    Pre-coated
    Protocol
    mouse CCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL9 has been precoated onto 96-well plates. Standards(E.coli, Q22-Q122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL9 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL9 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 34, Standard deviation: 1.19, CV(%): 3.5
    • Sample 2: n=16, Mean(pg/ml): 78, Standard deviation: 3.67, CV(%): 4.7
    • Sample 3: n=16, Mean(pg/ml): 294, Standard deviation: 14.99, CV(%): 5.1,
    • Sample 1: n=24, Mean(pg/ml): 35, Standard deviation: 1.68, CV(%): 4.8
    • Sample 2: n=24, Mean(pg/ml): 82, Standard deviation: 4.59, CV(%): 5.6
    • Sample 3: n=24, Mean(pg/ml): 302, Standard deviation: 19.03, CV(%): 6.3
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CCL9 (Ccl9) ELISA Kits
    CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
    Alternative Name
    Ccl9 (Ccl9 Products)
    Synonyms
    CCF18 ELISA Kit, MRP-2 ELISA Kit, Scya10 ELISA Kit, Scya9 ELISA Kit, chemokine (C-C motif) ligand 9 ELISA Kit, Ccl9 ELISA Kit
    Background

    Protein Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.

    Background: Chemokine(C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

    Synonyms: C-C motif chemokine 9,CCF18,Macrophage inflammatory protein 1-gamma,MIP-1-gamma,Macrophage inflammatory protein-related protein 2,MRP-2,Small-inducible cytokine A9,CCL9(29-101),CCL9(30-101),CCL9(31-101),Ccl9,Mrp2, Scya10, Scya9,

    Full Gene Name: C-C motif chemokine 9

    Cellular Localisation: Secreted.
    Gene ID
    20308
    UniProt
    P51670
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