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CD6 ELISA Kit

CD6 Reactivity: Human AA 18-398 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889385
  • Target See all CD6 ELISA Kits
    CD6
    Binding Specificity
    AA 18-398
    Reactivity
    • 5
    • 4
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10.000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human soluble CD6
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: H18-E398
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: H18-E398
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.

    Plate
    Pre-coated
    Protocol
    human CD6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD6 has been precoated onto 96-well plates. Standards(NSO, H18-E398) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD6 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD6 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.22, Standard deviation: 0.065, CV(%): 5.3
    • Sample 2: n=16, Mean(ng/ml): 3.17, Standard deviation: 0.149, CV(%): 4.7
    • Sample 3: n=16, Mean(ng/ml): 6.37, Standard deviation: 0.178, CV(%): 2.8,
    • Sample 1: n=24, Mean(ng/ml): 1.24, Standard deviation: 0.086, CV(%): 6.9
    • Sample 2: n=24, Mean(ng/ml): 3.24, Standard deviation: 0.204, CV(%): 6.3
    • Sample 3: n=24, Mean(ng/ml): 6.49, Standard deviation: 0.376, CV(%): 5.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CD6 ELISA Kits
    CD6
    Alternative Name
    CD6 (CD6 Products)
    Synonyms
    TP120 ELISA Kit, OX52 ELISA Kit, CD6 antigen ELISA Kit, CD6 molecule ELISA Kit, Cd6 molecule ELISA Kit, Cd6 ELISA Kit, CD6 ELISA Kit
    Background

    Protein Function: Involved in cell adhesion. Binds to CD166.

    Background: CD6(Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich(SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.

    Synonyms: T-cell differentiation antigen CD6,T12,TP120,CD6,CD6,

    Full Gene Name: T-cell differentiation antigen CD6

    Cellular Localisation: Cell membrane, Single-pass type I membrane protein.
    Gene ID
    923
    UniProt
    P30203
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