CD5 ELISA Kit

Catalog No. ABIN1889384

Product Details CD5 ELISA Kit

Target: CD5

Binding Specificity: AA 25-371

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.7-6000 pg/mL

Minimum Detection Limit: 93.7 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human soluble CD5

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: R25-N371

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: R25-N371

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: human CD5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD5 has been precoated onto 96-well plates. Standards(NSO, R25-N371) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD5 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human CD5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD5 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 0.37, Standard deviation: 0.023, CV(%): 6.2
• Sample 2: n=16, Mean(ng/ml): 0.85, Standard deviation: 0.041, CV(%): 4.8
• Sample 3: n=16, Mean(ng/ml): 4.27, Standard deviation: 0.167, CV(%): 3.9,
• Sample 1: n=24, Mean(ng/ml): 0.38, Standard deviation: 0.031, CV(%): 8.2
• Sample 2: n=24, Mean(ng/ml): 0.92, Standard deviation: 0.699, CV(%): 7.6
• Sample 3: n=24, Mean(ng/ml): 4.31, Standard deviation: 0.311, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CD5

Target: CD5

Alternative Name: CD5 (CD5 Products)

Synonyms: CD5 ELISA Kit, Ly-1 ELISA Kit, Ly-12 ELISA Kit, Ly-A ELISA Kit, Lyt-1 ELISA Kit, LEU1 ELISA Kit, T1 ELISA Kit, CD5 molecule ELISA Kit, CD5 antigen ELISA Kit, CD5 molecule like ELISA Kit, Cd5 molecule ELISA Kit, CD5 ELISA Kit, Cd5 ELISA Kit, CD5L ELISA Kit

Background:

Protein Function: May act as a receptor in regulating T-cell proliferation.

Background: CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli(such as bacterial proteins) and not by normal tissue proteins.

Synonyms: T-cell surface glycoprotein CD5,Lymphocyte antigen T1/Leu-1,CD5,CD5,LEU1,

Full Gene Name: T-cell surface glycoprotein CD5

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

Gene ID: 921

UniProt: P06127