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IL17RC ELISA Kit

IL17RC Reactivity: Mouse AA 22-440 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889355
  • Target See all IL17RC ELISA Kits
    IL17RC (Interleukin 17 Receptor C (IL17RC))
    Binding Specificity
    AA 22-440
    Reactivity
    • 5
    • 3
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10.000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17RC
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: E22-R440
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E22-R440
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    mouse IL-17RC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17RC has been precoated onto 96-well plates. Standards(NSO, E22-R440) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17RC amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IL-17RC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17RC standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 1458, Standard deviation: 61.24, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 4112, Standard deviation: 267.3, CV(%): 6.5
    • Sample 3: n=16, Mean(pg/ml): 6822, Standard deviation: 388.9, CV(%): 5.7,
    • Sample 1: n=24, Mean(pg/ml): 1632, Standard deviation: 88.13, CV(%): 5.4
    • Sample 2: n=24, Mean(pg/ml): 4473, Standard deviation: 335.5, CV(%): 7.5
    • Sample 3: n=24, Mean(pg/ml): 7188, Standard deviation: 488.8, CV(%): 6.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all IL17RC ELISA Kits
    IL17RC (Interleukin 17 Receptor C (IL17RC))
    Alternative Name
    IL17RC (IL17RC Products)
    Synonyms
    IL17-RL ELISA Kit, IL17RL ELISA Kit, 1110025H02Rik ELISA Kit, Gm19850 ELISA Kit, IL-17RC ELISA Kit, IL17-RC ELISA Kit, Il17rl ELISA Kit, interleukin 17 receptor C ELISA Kit, Il17rc ELISA Kit, IL17RC ELISA Kit
    Background

    Background: Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.

    Synonyms: Interleukin-17 receptor C,IL-17 receptor C,IL-17RC,Interleukin-17 receptor-like protein,IL-17RL,ZcytoR14,Il17rc,

    Full Gene Name: Interleukin-17 receptor C

    Cellular Localisation: Membrane, Single-pass type I membrane protein.
    Gene ID
    171095
    UniProt
    Q8K4C2
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