CD163 ELISA Kit

Catalog No. ABIN1889342

Product Details CD163 ELISA Kit

Target: CD163

Binding Specificity: AA 39-1045

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD163

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: V39-T1045

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <20pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: V39-T1045

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and mesenteric lymph nodes. .

Plate: Pre-coated

Protocol: mouse CD163 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD163 has been precoated onto 96-well plates. Standards(NSO, V39-T1045) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD163 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD163 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse CD163 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CD163 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 8.1, Standard deviation: 0.33, CV(%): 4.1
• Sample 2: n=16, Mean(ng/ml): 15.6, Standard deviation: 0.69, CV(%): 4.4
• Sample 3: n=16, Mean(ng/ml): 34, Standard deviation: 1.90, CV(%): 5.6,
• Sample 1: n=24, Mean(ng/ml): 9.2, Standard deviation: 0.57, CV(%): 6.2
• Sample 2: n=24, Mean(ng/ml): 17.8, Standard deviation: 1.01, CV(%): 5.7
• Sample 3: n=24, Mean(ng/ml): 32, Standard deviation: 1.70, CV(%): 5.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CD163

Target: CD163

Alternative Name: CD163 (CD163 Products)

Synonyms: DMBT1 ELISA Kit, CD163 ELISA Kit, CD163v2 ELISA Kit, CD163v3 ELISA Kit, M130 ELISA Kit, MM130 ELISA Kit, deleted in malignant brain tumors 1 protein-like ELISA Kit, CD163 molecule ELISA Kit, CD163 antigen ELISA Kit, LOC426820 ELISA Kit, CD163 ELISA Kit, Cd163 ELISA Kit

Background:

Protein Function: Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium- dependent and pH -dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity). .

Background: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

Synonyms: Scavenger receptor cysteine-rich type 1 protein M130,CD163,Soluble CD163,sCD163,Cd163,M130,

Full Gene Name: Scavenger receptor cysteine-rich type 1 protein M130

Cellular Localisation: Soluble CD163: Secreted.

Gene ID: 93671

UniProt: Q2VLH6