Kallikrein 6 ELISA Kit

Catalog No. ABIN1889322

Product Details Kallikrein 6 ELISA Kit

Target: Kallikrein 6 (KLK6)

Binding Specificity: AA 22-244

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78-5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallikrein-6

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: L22-K244

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <3pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: L22-K244

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

Tissue Specificity: In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors. .

Plate: Pre-coated

Protocol: human Kallikrein-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein-6 has been precoated onto 96-well plates. Standards(NSO, L22-K244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein-6 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Kallikrein-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Kallikrein-6 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 545, Standard deviation: 37.61, CV(%): 6.9
• Sample 2: n=16, Mean(pg/ml): 1711, Standard deviation: 80.42, CV(%): 4.7
• Sample 3: n=16, Mean(pg/ml): 2903, Standard deviation: 168.4, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 721, Standard deviation: 55.52, CV(%): 7.7
• Sample 2: n=24, Mean(pg/ml): 1927, Standard deviation: 104.1, CV(%): 5.4
• Sample 3: n=24, Mean(pg/ml): 3120, Standard deviation: 196.6, CV(%): 6.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Kallikrein 6 ELISA Kit (ABIN1889322)

Sells, Pandey, Chen, Skovan, Cui, Ignatenko: "Specific microRNA-mRNA Regulatory Network of Colon Cancer Invasion Mediated by Tissue Kallikrein-Related Peptidase 6." in: Neoplasia (New York, N.Y.), Vol. 19, Issue 5, pp. 396-411, (2018) (PubMed).

Target Details for Kallikrein 6

Target: Kallikrein 6 (KLK6)

Alternative Name: KLK6 (KLK6 Products)

Synonyms: Bssp ELISA Kit, Klk7 ELISA Kit, PRSS18 ELISA Kit, PRSS9 ELISA Kit, SP59 ELISA Kit, hK6 ELISA Kit, Prss9 ELISA Kit, AI849898 ELISA Kit, BSP ELISA Kit, Klk29 ELISA Kit, MSP ELISA Kit, Prss18 ELISA Kit, neurosin ELISA Kit, kallikrein related peptidase 6 ELISA Kit, kallikrein related-peptidase 6 ELISA Kit, kallikrein-6 ELISA Kit, KLK6 ELISA Kit, Klk6 ELISA Kit, LOC100716801 ELISA Kit

Background:

Protein Function: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. .

Background: KLK6(Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.

Synonyms: Kallikrein-6,3.4.21.-,Neurosin,Protease M,SP59,Serine protease 18,Serine protease 9,Zyme,KLK6,PRSS18, PRSS9,

Full Gene Name: Kallikrein-6

Cellular Localisation: Secreted. Nucleus, nucleolus. Cytoplasm. Mitochondrion. Microsome. In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress.

Gene ID: 5653

UniProt: Q92876

Pathways: Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling