Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

N-Cadherin ELISA Kit

CDH2 Reactivity: Human AA 160-724 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889308
  • Target See all N-Cadherin (CDH2) ELISA Kits
    N-Cadherin (CDH2) (Cadherin 2 (CDH2))
    Binding Specificity
    AA 160-724
    Reactivity
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.78-50 ng/mL
    Minimum Detection Limit
    0.78 ng/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cadherin-2/N-Cadherin
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: D160-A724
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <20pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: D160-A724
    Featured
    Discover our best selling CDH2 ELISA Kit
    Top Product
    Discover our top product CDH2 ELISA Kit
  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    human Cadherin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cadherin-2 has been precoated onto 96-well plates. Standards(NSO, D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cadherin-2 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Cadherin-2 standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 9.6, Standard deviation: 0.365, CV(%): 3.8
    • Sample 2: n=16, Mean(ng/ml): 22.4, Standard deviation: 0.963, CV(%): 4.3
    • Sample 3: n=16, Mean(ng/ml): 38.2, Standard deviation: 1.986, CV(%): 5.2,
    • Sample 1: n=24, Mean(ng/ml): 12.5, Standard deviation: 0.675, CV(%): 5.4
    • Sample 2: n=24, Mean(ng/ml): 26.4, Standard deviation: 1.61, CV(%): 6.1
    • Sample 3: n=24, Mean(ng/ml): 40.7, Standard deviation: 2.768, CV(%): 6.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all N-Cadherin (CDH2) ELISA Kits
    N-Cadherin (CDH2) (Cadherin 2 (CDH2))
    Alternative Name
    CDH2 (CDH2 Products)
    Synonyms
    CD325 ELISA Kit, CDHN ELISA Kit, CDw325 ELISA Kit, NCAD ELISA Kit, N-cadherin ELISA Kit, cadherin-2 ELISA Kit, cdhn ELISA Kit, ncad ELISA Kit, cdw325 ELISA Kit, CDH2 ELISA Kit, Cadherin-2 ELISA Kit, Ncad ELISA Kit, glo ELISA Kit, lyr ELISA Kit, pac ELISA Kit, wu:fb47h04 ELISA Kit, cadherin 2 ELISA Kit, cadherin 2 S homeolog ELISA Kit, cadherin 2, type 1, N-cadherin (neuronal) ELISA Kit, CDH2 ELISA Kit, cdh2.S ELISA Kit, cdh2 ELISA Kit, Cdh2 ELISA Kit
    Background

    Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). .

    Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

    Synonyms: Cadherin-2,CDw325,Neural cadherin,N-cadherin,CD325,CDH2,CDHN, NCAD,

    Full Gene Name: Cadherin-2

    Cellular Localisation: Cell membrane, Single-pass type I membrane protein.
    Gene ID
    1000
    UniProt
    P19022
    Pathways
    Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
You are here:
Support