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IGFBP2 ELISA Kit

IGFBP2 Reactivity: Mouse AA 35-305 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889295
  • Target See all IGFBP2 ELISA Kits
    IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
    Binding Specificity
    AA 35-305
    Reactivity
    • 10
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    125-8000 pg/mL
    Minimum Detection Limit
    125 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP2
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: E35-Q305
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E35-Q305
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 IGFBP N-terminal domain.

    Tissue Specificity: Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary. .

    Plate
    Pre-coated
    Protocol
    mouse IGFBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP2 has been precoated onto 96-well plates. Standards(NSO, E35-Q305) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP2 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IGFBP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IGFBP2 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 1257, Standard deviation: 89.2, CV(%): 7.1
    • Sample 2: n=16, Mean(pg/ml): 3204, Standard deviation: 166.6, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 5394, Standard deviation: 350.61, CV(%): 6.5,
    • Sample 1: n=24, Mean(pg/ml): 1020, Standard deviation: 84.66, CV(%): 8.3
    • Sample 2: n=24, Mean(pg/ml): 3562, Standard deviation: 239, CV(%): 6.7
    • Sample 3: n=24, Mean(pg/ml): 4975, Standard deviation: 368.15, CV(%): 7.4
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all IGFBP2 ELISA Kits
    IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
    Alternative Name
    IGFBP2 (IGFBP2 Products)
    Synonyms
    IBP2 ELISA Kit, IGF-BP53 ELISA Kit, AI255832 ELISA Kit, IBP-2 ELISA Kit, Igfbp-2 ELISA Kit, mIGFBP-2 ELISA Kit, IGFBP-2 ELISA Kit, ILGFBPA ELISA Kit, pIGFBP-2 ELISA Kit, igfbp2 ELISA Kit, MGC65741 ELISA Kit, MGC76823 ELISA Kit, MGC174445 ELISA Kit, IGFBP2 ELISA Kit, ibp2 ELISA Kit, igf-bp53 ELISA Kit, igfbp2a ELISA Kit, si:ch211-2k18.3 ELISA Kit, insulin like growth factor binding protein 2 ELISA Kit, insulin-like growth factor binding protein 2 ELISA Kit, insulin-like growth factor binding protein 2a ELISA Kit, insulin-like growth factor binding protein 2b ELISA Kit, IGFBP2 ELISA Kit, Igfbp2 ELISA Kit, igfbp2a ELISA Kit, igfbp2 ELISA Kit, igfbp2b ELISA Kit
    Background

    Protein Function: Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. .

    Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

    Synonyms: Insulin-like growth factor-binding protein 2,IBP-2,IGF-binding protein 2,IGFBP-2,mIGFBP-2,Igfbp2,Igfbp-2,

    Full Gene Name: Insulin-like growth factor-binding protein 2

    Cellular Localisation: Secreted.
    Gene ID
    16008
    UniProt
    P47877
    Pathways
    Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation
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