SensoLyte® 520 Thrombin Activity Assay Kit

Catalog No. ABIN1882567

Product Details

Target: alpha-Thrombin

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity. This kit contains a novel internally quenched 5-FAM/QXL™ 520 FRET substrate for thrombin. Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.

Storage: -80 °C

Storage Comment: Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Components C and F can be stored at room temperature for convenience.

References

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Target Details

Target: alpha-Thrombin

Alternative Name: Thrombin (alpha-Thrombin Products)

Synonyms: PT Kit, RPRGL2 Kit, THPH1 Kit, coagulation factor II, thrombin Kit, F2 Kit

Background: Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.