SensoLyte® Thiol Quantitation Assay Kit

Catalog No. ABIN1882564

Product Details

Target: Thiol

Detection Method: Colorimetric

Application: Enzyme Activity Assay (EAA)

Brand: SensoLyte®

Characteristics: The SensoLyte® Thiol Quantitation Assay Kit utilizes the widely used Elmann's reagent for colorimetric measurement of thiol concentrations. The sulfhydryl group of thiol reacts with Ellman's Reagent [5, 5'-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to thiol concentration.

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Component A from light and moisture.

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Component C can be stored at room temperature for convenience.

Target Details

Target: Thiol

Background: Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators.