SensoLyte® Green SIRT2 Assay Kit

Catalog No. ABIN1882555

Product Details

Target: SIRT2 (Sirtuin 2 (SIRT2))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® Green SIRT2 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 2 activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with SIRT2 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step, mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. This kit can be used for high throughput screening (HTS) of SIRT2 inhibitors/activators. The long wavelength fluorescence of SIRT2 substrate is less interfered by the autofluorescence of cell components and test compounds. Assays are performed in a standard 96-well plate format. 100 assays (96-well plate)

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.

Storage: -80 °C

Storage Comment: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

Target Details

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2 (SIRT2 Products)

Synonyms: CG5085 Kit, D.mel2 Kit, Dmel\\CG5085 Kit, SIRT2 Kit, dSIRT2 Kit, sir2 Kit, Sirt2 Kit, BmSirt2 Kit, AtSRT2 Kit, SIRTUIN 2 Kit, T2K12.8 Kit, sirtuin 2 Kit, SIR2 Kit, SIR2L Kit, SIR2L2 Kit, 5730427M03Rik Kit, Sir2l Kit, wu:fb57d05 Kit, zgc:55966 Kit, zgc:77003 Kit, Sirtuin 2 Kit, sirtuin 2 Kit, sirtuin 2 S homeolog Kit, NAD-dependent deacetylase sirtuin 2 homolog Kit, sirtuin 2 (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) Kit, Sirt2 Kit, SIRT2 Kit, sirt2.S Kit, SRT2 Kit, sirt2 Kit

Background: Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins. Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD+ as a cofactor. Sirtuin 2 (SIRT2) is a cytoplasmic protein involved in regulation of cell cycle, DNA repair and tumorogenesis.