Target See all SIRT1 Kits: SIRT1 (Sirtuin 1 (SIRT1))
Detection Method: Fluorometric
Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)
Brand: SensoLyte®
Characteristics: The SensoLyte® 520 FRET SIRT1 Assay Kit provides a convenient, two-step homogeneous procedure for measuring sirtuin 1 activity using FRET substrate. In the first step, an acetylated substrate is incubated with SIRT1 containing samples. Deacetylation of substrate first sensitizes it, so that, in the second step mixing with the SIRT developer generates a fluorophore, which can then be detected at Ex/Em= 490/520 nm. This kit can be used for high throughput screening of SIRT1 inhibitors/activators. The long wavelength fluorescence of FRET substrate is less interfered by the autofluorescence of cell components and test compounds. The nuclear extract HDAC (containing HDACs 1 and 2) does not deacetylate FRET sirtuin substrate. Assays are performed in a standard 96-well format.
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Sirtuin 1 (SIRT1) ELISA Kit

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Sirtuin 1 (SIRT1) ELISA Kit

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SIRT1 Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

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SIRT1 Reactivity: Human qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum

Sirtuin 1 (SIRT1) ELISA Kit

SIRT1 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Sirtuin 1 (SIRT1) ELISA Kit

SIRT1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate

Handling Advice: Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.
Storage: -80 °C
Storage Comment: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.

Target: SIRT1 (Sirtuin 1 (SIRT1))
Alternative Name: Sirt1 (SIRT1 Products)
Synonyms: sirtuin 1 Kit, AA673258 Kit, SIR2L1 Kit, Sir2 Kit, Sir2a Kit, Sir2alpha Kit, sir2l1 Kit, sirtuin Kit, SIR2 Kit, sirtuin 1 Kit, sirtuin 1 L homeolog Kit, SIRT1 Kit, SRT1 Kit, Sirt1 Kit, sirt1.L Kit
Background: Histone deacetylase (HDAC) enzymes act as transcriptional repressors of genes through the deacetylation of lysine residues on histone proteins. Sirtuins (SIRTs) are a unique class of deacetylases (class III HDACs) that require NAD as a cofactor. Sirtuin 1 (SIRT1) is involved in regulation of tumor suppressor p53 and inhibition of apoptosis. It also represents a target for treatment of age-related diseases and type II diabetes.

SensoLyte® 520 FRET Sirt1 Assay Kit

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