SensoLyte® 520 Renin Assay Kit

Catalog No. ABIN1882550

Product Details

Target: Renin (REN)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL™520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay and can detect 0.8 ng/mL renin.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -80 °C

Storage Comment: Store all kit components at -20 °C expect for Component C, which should be stored at -80 °C. If used frequently, Component D can be stored at room temperature for convenience.

References

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May, Dean, Lowe, Martenyi, Sheehan, Boggs, Monk, Mathes, Mergott, Watson, Stout, Timm, Smith Labell, Gonzales, Nakano, Jhee, Yen, Ereshefsky, Lindstrom, Calligaro, Cocke, Greg Hall, Friedrich, Citron et al.: "Robust central reduction of amyloid-? in humans with an orally available, non-peptidic ?-secretase inhibitor. ..." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 46, pp. 16507-16, (2011) (PubMed).

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Yoshikawa, Aizaki, Kusano, Kishi, Susumu, Iida, Ishiura, Nishimura, Shichiri, Senbonmatsu: "The (pro)renin receptor is cleaved by ADAM19 in the Golgi leading to its secretion into extracellular space." in: Hypertension research : official journal of the Japanese Society of Hypertension, Vol. 34, Issue 5, pp. 599-605, (2011) (PubMed).

Iwai, Kanno, Tomono, Inaba, Senba, Furuno, Mogi, Horiuchi: "Direct renin inhibition improved insulin resistance and adipose tissue dysfunction in type 2 diabetic KK-A(y) mice." in: Journal of hypertension, Vol. 28, Issue 7, pp. 1471-81, (2010) (PubMed).

Feliers, Kasinath: "Mechanism of VEGF expression by high glucose in proximal tubule epithelial cells." in: Molecular and cellular endocrinology, Vol. 314, Issue 1, pp. 136-42, (2009) (PubMed).

Schmiedt, Hurley, Tong, Rakhmanova, Po, Hurley: "Measurement of plasma renin concentration in cats by use of a fluorescence resonance energy transfer peptide substrate of renin." in: American journal of veterinary research, Vol. 70, Issue 11, pp. 1315-22, (2009) (PubMed).

Wei, Ma, Hattori: "Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases." in: Bioorganic & medicinal chemistry, Vol. 17, Issue 8, pp. 3003-10, (2009) (PubMed).

Zivná, H?lková, Matignon, Hodanová, Vyletal, Kalbácová, Baresová, Sikora, Blazková, Zivný, Ivánek, Stránecký, Sovová, Claes, Lerut, Fryns, Hart, Hart, Adams, Pawtowski, Clemessy, Gasc, Gübler et al.: "Dominant renin gene mutations associated with early-onset hyperuricemia, anemia, and chronic kidney failure. ..." in: American journal of human genetics, Vol. 85, Issue 2, pp. 204-13, (2009) (PubMed).

Target Details

Target: Renin (REN)

Alternative Name: Renin (REN Products)

Synonyms: HNFJ2 Kit, RATRENAA Kit, RENAA Kit, Ren1 Kit, D19352 Kit, Ren Kit, Ren-1 Kit, Ren-A Kit, Ren1c Kit, Ren1d Kit, Rn-1 Kit, Rnr Kit, renin Kit, renin 1 structural Kit, REN Kit, Ren Kit, Ren1 Kit, ren Kit

Background: Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease.