SensoLyte® Rh110 Plasmin Activity Assay Kit

Catalog No. ABIN1882542

Product Details

Target: Plasmin (Plasmin/fibrinolysin (PL/Fbn))

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® Rh110 Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from reaction components.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Storage: -80 °C

Storage Comment: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Protect Components A and B from light and moisture. Components D and F can be stored at room temperature for convenience.

References

Ikezoe, Yang, Nishioka, Isaka, Iwabu, Sakai, Taniguchi, Honda, Yokoyama: "Thrombomodulin enhances the antifibrinolytic and antileukemic effects of all-trans retinoic acid in acute promyelocytic leukemia cells." in: Experimental hematology, Vol. 40, Issue 6, pp. 457-65, (2012) (PubMed).

Target Details

Target: Plasmin (Plasmin/fibrinolysin (PL/Fbn))

Alternative Name: Plasmin (Plasmin Products)

Synonyms: plasminogen Kit, PLG Kit

Background: Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis.