SensoLyte® Plus 520 MMP-13 Assay Kit Fluorimetric and Enhanced Selectivity

Catalog No. ABIN1882517

Product Details

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity: Human

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® Plus 520 MMP-13 Assay Kit is designed for specifically detecting MMP-13 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-13 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-13-induced cleavage of the FRET substrate.

Application Details

Comment:

ELISA + FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Storage: -80 °C

Storage Comment: Store all kit components, except Component B, at -20 °C. Store Component B at -80 °C. For convenience, Components D, G, H, I can be stored at RT.

References

Jackson, Moradi, Smith, Jackson, Little: "Activation of matrix metalloproteinases 2, 9, and 13 by activated protein C in human osteoarthritic cartilage chondrocytes." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 66, Issue 6, pp. 1525-36, (2014) (PubMed).

Ozeki, Kawai, Yamaguchi, Hiyama, Kinoshita, Hase, Nakata, Kondo, Mogi, Nakamura: "IL-1?-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells." in: Experimental cell research, Vol. 323, Issue 1, pp. 165-77, (2014) (PubMed).

Mak, Turcotte, Popovic, Singh, Ghert: "AP-1 as a Regulator of MMP-13 in the Stromal Cell of Giant Cell Tumor of Bone." in: Biochemistry research international, Vol. 2011, pp. 164197, (2011) (PubMed).

Cowan, Mak, Colterjohn, Singh, Ghert: "Collagenase expression and activity in the stromal cells from giant cell tumour of bone." in: Bone, Vol. 44, Issue 5, pp. 865-71, (2009) (PubMed).

Moor, Vachon, Gould: "Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers." in: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 17, Issue 6, pp. 832-9, (2009) (PubMed).

Salinas, Anseth: "The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities." in: Biomaterials, Vol. 29, Issue 15, pp. 2370-7, (2008) (PubMed).

Bai, Merchant: "A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion." in: FEBS letters, Vol. 581, Issue 30, pp. 5904-10, (2007) (PubMed).

Target Details

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP-13 (MMP13 Products)

Synonyms: CLG3 Kit, MANDP1 Kit, Clg Kit, MMP-13 Kit, Mmp1 Kit, cb1034 Kit, mmp13 Kit, gene A Kit, xCol Kit, xcl3 Kit, MMP13 Kit, MGC108008 Kit, matrix metallopeptidase 13 Kit, matrix metallopeptidase 13a Kit, matrix metallopeptidase 13 (collagenase 3) like S homeolog Kit, matrix metallopeptidase 13 (collagenase 3) Kit, matrix metalloproteinase 13 Kit, matrix metallopeptidase 13 (collagenase 3) like L homeolog Kit, MMP13 Kit, Mmp13 Kit, mmp13a Kit, mmp13l.S Kit, mmp13 Kit, LOC100136348 Kit, mmp13l.L Kit