SensoLyte® 520 MMP-13 Assay Kit

Catalog No. ABIN1882516

Product Details

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: SensoLyte® 520 MMP-13 Assay Kit uses a 5-FAM (fluorophore) and QXL™ 520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-13, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™ 520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

References

Wang, Sampson, Jin, Li, Ke, Im, Chen: "MMP13 is a critical target gene during the progression of osteoarthritis." in: Arthritis research & therapy, Vol. 15, Issue 1, pp. R5, (2014) (PubMed).

Wigner, Kulkarni, Yakavonis, Young, Tinsley, Meeks, Einhorn, Gerstenfeld: "Urine matrix metalloproteinases (MMPs) as biomarkers for the progression of fracture healing." in: Injury, Vol. 43, Issue 3, pp. 274-8, (2012) (PubMed).

Bedi, Fox, Kovacevic, Deng, Warren, Rodeo: "Doxycycline-mediated inhibition of matrix metalloproteinases improves healing after rotator cuff repair." in: The American journal of sports medicine, Vol. 38, Issue 2, pp. 308-17, (2010) (PubMed).

Madala, Pesce, Ramalingam, Wilson, Minnicozzi, Cheever, Thompson, Mentink-Kane, Wynn: "Matrix metalloproteinase 12-deficiency augments extracellular matrix degrading metalloproteinases and attenuates IL-13-dependent fibrosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 7, pp. 3955-63, (2010) (PubMed).

Target Details

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP-13 (MMP13 Products)

Synonyms: CLG3 Kit, MANDP1 Kit, Clg Kit, MMP-13 Kit, Mmp1 Kit, cb1034 Kit, mmp13 Kit, gene A Kit, xCol Kit, xcl3 Kit, MMP13 Kit, MGC108008 Kit, matrix metallopeptidase 13 Kit, matrix metallopeptidase 13a Kit, matrix metallopeptidase 13 (collagenase 3) like S homeolog Kit, matrix metallopeptidase 13 (collagenase 3) Kit, matrix metalloproteinase 13 Kit, matrix metallopeptidase 13 (collagenase 3) like L homeolog Kit, MMP13 Kit, Mmp13 Kit, mmp13a Kit, mmp13l.S Kit, mmp13 Kit, LOC100136348 Kit, mmp13l.L Kit

Background: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-13 (collagenase-3, rat collagenase) is involved in quite a few diseases such as arthritis and asthma.