SensoLyte® 490 MMP-12 Assay Kit

Catalog No. ABIN1882513

Product Details

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 490 MMP-12 Assay Kit is a complete assay system designed to continuously analyze MMP-12 activity or to screen for MMP-12 inhibitors by using a fluorogenic MMP-12 substrate.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

References

Song, Xie, Liu, Zhao, Yu, Ban, Ye, Wen, Wan, Li, Ma, Meng: "Expression of matrix metalloproteinase-12 in aortic dissection." in: BMC cardiovascular disorders, Vol. 13, pp. 34, (2013) (PubMed).

Chan, Chan, Yeung, Man, Ip, Mak: "Inhibitory effect of Chinese green tea on cigarette smoke-induced up-regulation of airway neutrophil elastase and matrix metalloproteinase-12 via antioxidant activity." in: Free radical research, Vol. 46, Issue 9, pp. 1123-9, (2012) (PubMed).

Bogaert, Naessens, De Koker, Hennuy, Hacha, Smet, Cataldo, Di Valentin, Piette, Tournoy, Grooten: "Inflammatory signatures for eosinophilic vs. neutrophilic allergic pulmonary inflammation reveal critical regulatory checkpoints." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 300, Issue 5, pp. L679-90, (2011) (PubMed).

Qu, Du, Wang, Yan: "Matrix metalloproteinase 12 overexpression in lung epithelial cells plays a key role in emphysema to lung bronchioalveolar adenocarcinoma transition." in: Cancer research, Vol. 69, Issue 18, pp. 7252-61, (2009) (PubMed).

Takiyama, Kinoshita, Kinoshita-Kikuta, Fujioka, Kubo, Koike: "A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptides." in: Analytical biochemistry, Vol. 388, Issue 2, pp. 235-41, (2009) (PubMed).

Ma, Tittiger, Knutsen, Kovacs, Schaller, Mecham, Ponder: "Upregulation of elastase proteins results in aortic dilatation in mucopolysaccharidosis I mice." in: Molecular genetics and metabolism, Vol. 94, Issue 3, pp. 298-304, (2008) (PubMed).

Summer, Little, Ouchi, Takemura, Aprahamian, Dwyer, Fitzsimmons, Suki, Parameswaran, Fine, Walsh: "Alveolar macrophage activation and an emphysema-like phenotype in adiponectin-deficient mice." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 294, Issue 6, pp. L1035-42, (2008) (PubMed).

Demedts, Morel-Montero, Lebecque, Pacheco, Cataldo, Joos, Pauwels, Brusselle: "Elevated MMP-12 protein levels in induced sputum from patients with COPD." in: Thorax, Vol. 61, Issue 3, pp. 196-201, (2006) (PubMed).

Target Details

Target: MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))

Alternative Name: MMP-12 (MMP12 Products)

Synonyms: MMP12 Kit, AV378681 Kit, Mmel Kit, Mme Kit, HME Kit, ME Kit, MME Kit, MMP-12 Kit, matrix metallopeptidase 12 Kit, MMP12 Kit, Mmp12 Kit

Background: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is implicated in the pathology of many diseases such as emphysema, aortic lesions and cancer. It has recently been shown to play a role in the pathogenesis of rheumatoid arthritis.