SensoLyte® 520 MMP-1 Assay Kit

Catalog No. ABIN1882510

Product Details

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: SensoLyte® 520 MMP-1 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrate for continuous measurement of enzyme activity. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-1, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.

References

Herrera, Cisneros, Maldonado, Ramírez, Ortiz-Quintero, Anso, Chandel, Selman, Pardo: "Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 25964-75, (2013) (PubMed).

Dayoub, Wagner, Bataille, Stöltzing, Spruss, Buechler, Schlitt, Weiss: "Liver regeneration associated protein (ALR) exhibits antimetastatic potential in hepatocellular carcinoma." in: Molecular medicine (Cambridge, Mass.), Vol. 17, Issue 3-4, pp. 221-8, (2011) (PubMed).

Yang, Lee, Choi, Lee, Kim, Shin, Han, Kang, Chung: "Matrix metalloproteinase-1 induces cleavage of exogenous alphaB-crystallin transduced by a cell-penetrating peptide." in: Journal of cellular biochemistry, Vol. 112, Issue 9, pp. 2454-62, (2011) (PubMed).

Spiga, Borrás: "Development of a gene therapy virus with a glucocorticoid-inducible MMP1 for the treatment of steroid glaucoma." in: Investigative ophthalmology & visual science, Vol. 51, Issue 6, pp. 3029-41, (2010) (PubMed).

Yi, Zhang, Li, Muh, Fillet, Renert, Li: "Inhibition of ceramide-redox signaling pathway blocks glomerular injury in hyperhomocysteinemic rats." in: Kidney international, Vol. 70, Issue 1, pp. 88-96, (2006) (PubMed).

Target Details

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Alternative Name: MMP-1 (MMP1 Products)

Synonyms: CLG Kit, CLGN Kit, Mmp1a Kit, CG4859 Kit, Dm1-MMP Kit, Dmel\\CG4859 Kit, MMP-1 Kit, MMP1 Kit, Mmp 1 Kit, dMMP1 Kit, dm1-MMP Kit, dmmp1 Kit, l(2)k04809 Kit, mmp1 Kit, Mmp1 Kit, Mcol-A Kit, Mcola Kit, col4 Kit, mmp18 Kit, Clgn Kit, matrix metallopeptidase 1 Kit, Matrix metalloproteinase 1 Kit, matrix metalloproteinase 1 Kit, matrix metallopeptidase 1 (interstitial collagenase) Kit, matrix metallopeptidase 1a (interstitial collagenase) Kit, matrix metallopeptidase 1 S homeolog Kit, interstitial collagenase Kit, matrix metallopeptidase 8 L homeolog Kit, MMP1 Kit, Mmp1 Kit, RB11133 Kit, Mmp1a Kit, mmp1.S Kit, LOC100727966 Kit, mmp8.L Kit

Background: MMP-1 (interstitial collagenase, fibroblast collagenase) is an extracellular protease. This enzyme plays a significant role in cancer, arthritis and other diseases, and has been identified as a drug-screening target.