SensoLyte® 520 HDAC Activity Assay Kit

Catalog No. ABIN1882477

Product Details

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with HDAC containing samples. Deacetylation of substrate sensitizes it, so that, in the second step mixing with the HDAC developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. The substrate included in the kit is cell-permeable, and the assay can be used to measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. This kit also can be used for high throughput screening of HDAC inhibitors using extracts or purified enzymes. The long wavelength fluorescence of HDAC 520 substrate is less interfered by the autofluorescence of cell components and test compounds.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.

Storage: -80 °C

Storage Comment: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Components D and G can be stored at room temperature for convenience.

References

Park, Ahn, Kim, Yoon, Kang, Lee, Moon, Jung, Chung, Kim: "A new synthetic HDAC inhibitor, MHY218, induces apoptosis or autophagy-related cell death in tamoxifen-resistant MCF-7 breast cancer cells." in: Investigational new drugs, Vol. 30, Issue 5, pp. 1887-98, (2012) (PubMed).

Ehnert, Zhao, Pscherer, Freude, Dooley, Kolk, Stöckle, Nussler, Hube: "Transforming growth factor ?1 inhibits bone morphogenic protein (BMP)-2 and BMP-7 signaling via upregulation of Ski-related novel protein N (SnoN): possible mechanism for the failure of BMP therapy?" in: BMC medicine, Vol. 10, pp. 101, (2012) (PubMed).

Kang, Kim, Ko: "A novel isoform of human LZIP negatively regulates the transactivation of the glucocorticoid receptor." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 11, pp. 1746-57, (2009) (PubMed).

Target Details

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC (HDAC1 Products)

Synonyms: GON-10 Kit, HD1 Kit, RPD3 Kit, RPD3L1 Kit, CG7471 Kit, DHDAC1 Kit, DRpd3 Kit, DmHDAC1 Kit, Dmel\\CG7471 Kit, E(var)3-64BC Kit, HDAC Kit, HDAC-1 Kit, HDAC1 Kit, Hdac1 Kit, Rpd3/HDAC Kit, Su(var)3-26 Kit, Su(var)326 Kit, Su(var)328 Kit, dHDAC-1 Kit, dHDAC1 Kit, dRPD3 Kit, dRpd3 Kit, dmHDA401 Kit, drpd3 Kit, hdac1 Kit, l(3)04556 Kit, l(3)64Cc Kit, rpd3 Kit, rpd[3] Kit, gon-10 Kit, hdac1b Kit, rpd3l1 Kit, Rpd3 Kit, GB14706 Kit, hdac1-b Kit, chunp6919 Kit, hdac-1 Kit, mp:zf637-2-001987 Kit, wu:fb19h11 Kit, wu:fi06f03 Kit, zgc:101582 Kit, zgc:65818 Kit, Hdac1-ps Kit, MommeD5 Kit, ARABIDOPSIS HISTONE DEACETYLASE 1 Kit, ARABIDOPSIS HISTONE DEACETYLASE 19 Kit, ATHD1 Kit, ATHDA19 Kit, ATRPD3A Kit, F20D10.250 Kit, F20D10_250 Kit, HDA1 Kit, HDA19 Kit, HISTONE DEACETYLASE Kit, HISTONE DEACETYLASE 19 Kit, HISTONE DEACETYLASE19 Kit, RPD3A Kit, histone deacetylase 1 Kit, HDM Kit, ab21 Kit, hdac1a Kit, histone deacetylase 1 Kit, Histone deacetylase 1 Kit, histone deacetylase Kit, histone deacetylase 1 S homeolog Kit, histone deacetylase Rpd3 Kit, histone deacetylase 1 L homeolog Kit, HDAC1 Kit, hdac1.S Kit, LOC411503 Kit, hdac1 Kit, Hdac1 Kit, HD1 Kit, hda-1 Kit, hdac1.L Kit, LOC748850 Kit

Background: Histone deacetylase (HDAC) enzymes modulate gene expression through the deacetylation of lysine residues on histone proteins and act as transcriptional repressors of genes. Based on their role in cell cycling, apoptosis and differentiation, HDACs have been chosen as therapeutic targets for the treatment of cancer and neurodegenerative diseases.