SensoLyte® 520 HDAC Activity Assay Kit

Catalog No. ABIN1882477

Product Details

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a fluorogenic substrate. In the first step, an acetylated substrate is incubated with HDAC containing samples. Deacetylation of substrate sensitizes it, so that, in the second step mixing with the HDAC developer generates a fluorophore, which can then be detected at Ex/Em= 490 nm/520 nm. The substrate included in the kit is cell-permeable, and the assay can be used to measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. This kit also can be used for high throughput screening of HDAC inhibitors using extracts or purified enzymes. The long wavelength fluorescence of HDAC 520 substrate is less interfered by the autofluorescence of cell components and test compounds.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Aliquot as needed to avoid freeze-thaw cycles. Protect Components A and B from light and from moisture.

Storage: -80 °C

Storage Comment: Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Components D and G can be stored at room temperature for convenience.

References

Park, Ahn, Kim, Yoon, Kang, Lee, Moon, Jung, Chung, Kim: "A new synthetic HDAC inhibitor, MHY218, induces apoptosis or autophagy-related cell death in tamoxifen-resistant MCF-7 breast cancer cells." in: Investigational new drugs, Vol. 30, Issue 5, pp. 1887-98, (2012) (PubMed).

Ehnert, Zhao, Pscherer, Freude, Dooley, Kolk, Stöckle, Nussler, Hube: "Transforming growth factor ?1 inhibits bone morphogenic protein (BMP)-2 and BMP-7 signaling via upregulation of Ski-related novel protein N (SnoN): possible mechanism for the failure of BMP therapy?" in: BMC medicine, Vol. 10, pp. 101, (2012) (PubMed).

Kang, Kim, Ko: "A novel isoform of human LZIP negatively regulates the transactivation of the glucocorticoid receptor." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 11, pp. 1746-57, (2009) (PubMed).

Target Details

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC (HDAC1 Products)

Background: Histone deacetylase (HDAC) enzymes modulate gene expression through the deacetylation of lysine residues on histone proteins and act as transcriptional repressors of genes. Based on their role in cell cycling, apoptosis and differentiation, HDACs have been chosen as therapeutic targets for the treatment of cancer and neurodegenerative diseases.