SensoLyte® 520 HCV Protease Assay Kit

Catalog No. ABIN1882473

Product Details

Target: Hepatitis C Virus (HCV)

Reactivity: Hepatitis C Virus (HCV)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL™ 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at:
Excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL™ 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Components C and D can be stored at room temperature for convenience.

References

Furuta, Salam, Hermawan, Akimitsu, Tanaka, Tani, Yamashita, Moriishi, Nakakoshi, Tsubuki, Peng, Suzuki, Yamamoto, Sekiguchi, Tsuneda, Noda: "Identification and biochemical characterization of halisulfate 3 and suvanine as novel inhibitors of hepatitis C virus NS3 helicase from a marine sponge." in: Marine drugs, Vol. 12, Issue 1, pp. 462-76, (2014) (PubMed).

Salah El Dine, Abdel Monem, El-Halawany, Hattori, Abdel-Sattar: "HCV-NS3/4A protease inhibitory iridoid glucosides and dimeric foliamenthoic acid derivatives from Anarrhinum orientale." in: Journal of natural products, Vol. 74, Issue 5, pp. 943-8, (2011) (PubMed).

Salim, Aoyama, Sugita, Watashi, Wakita, Hamasaki, Okamoto, Urata, Hashimoto, Baba: "Potent and selective inhibition of hepatitis C virus replication by novel phenanthridinone derivatives." in: Biochemical and biophysical research communications, Vol. 415, Issue 4, pp. 714-9, (2011) (PubMed).

Ma, Wu, Masao, Wang, Kano: "HCV protease inhibitory, cytotoxic and apoptosis-inducing effects of oleanolic acid derivatives." in: Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Société canadienne des sciences pharmaceutiques, Vol. 12, Issue 3, pp. 243-8, (2010) (PubMed).

Phuong, Ma, Hattori, Jin: "Inhibitory effects of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease." in: Phytotherapy research : PTR, Vol. 23, Issue 4, pp. 582-4, (2009) (PubMed).

Wang, Tseng, Tsai, Lin, Wen, Tsay, Sakamoto, Tseng, Cheng: "Bioactivity-guided screening identifies pheophytin a as a potent anti-hepatitis C virus compound from Lonicera hypoglauca Miq." in: Biochemical and biophysical research communications, Vol. 385, Issue 2, pp. 230-5, (2009) (PubMed).

Wei, Ma, Hattori: "Synthesis of dammarane-type triterpene derivatives and their ability to inhibit HIV and HCV proteases." in: Bioorganic & medicinal chemistry, Vol. 17, Issue 8, pp. 3003-10, (2009) (PubMed).

Ma, Wei, Wang, Hattori: "Triterpenes from Cynomorium songaricium--analysis of HCV protease inhibitory activity, quantification, and content change under the influence of heating." in: Journal of natural medicines, Vol. 63, Issue 1, pp. 9-14, (2008) (PubMed).

Ma, Yates, Liang, Lemon, Yi: "NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly." in: Journal of virology, Vol. 82, Issue 15, pp. 7624-39, (2008) (PubMed).

Target Details

Target: Hepatitis C Virus (HCV)

Abstract: HCV Products

Target Type: Virus