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- Target See all Fluorescein Diphosphate (FDP) products
- Fluorescein Diphosphate (FDP)
- Detection Method
- Fluorometric
- Application
- Enzyme Activity Assay (EAA)
- Brand
- SensoLyte®
- Characteristics
- SensoLyte® FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). The kit contains: FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation), Calibration standard, Reaction buffer, Stop buffer, An optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments
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- Comment
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Enzymatic Activity Assay kit
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- Store Component A at -20 °C. Store other kit components at 4 °C.
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Effects of pseudowollastonite (CaSiO3) bioceramic on in vitro activity of human mesenchymal stem cells." in: Biomaterials, Vol. 31, Issue 30, pp. 7653-65, (2010) (PubMed).
: "Nerve growth factor promotes differentiation of odontoblast-like cells." in: Journal of cellular biochemistry, Vol. 106, Issue 4, pp. 539-45, (2009) (PubMed).
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Effects of pseudowollastonite (CaSiO3) bioceramic on in vitro activity of human mesenchymal stem cells." in: Biomaterials, Vol. 31, Issue 30, pp. 7653-65, (2010) (PubMed).
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- Target
- Fluorescein Diphosphate (FDP)
- Alternative Name
- FDP (FDP Products)
- Background
- Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses.
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