SensoLyte® Rh110 Factor Xa Assay Kit
-
- Target See all Factor Xa (FXa) Kits
- Factor Xa (FXa) (Activated Coagulation Factor X (FXa))
- Detection Method
- Fluorometric
- Application
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- Brand
- SensoLyte®
- Characteristics
- The SensoLyte® Rh110 Factor Xa Assay Kit provides a convenient assay for screening of FXa inhibitors or continuous assay of enzyme activity using a fluorogenic substrate. Upon FXa protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore which has a bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components.
- Top Product
- Discover our top product FXa ELISA Kit
-
-
- Comment
-
FRET-based Assay Kit
- Restrictions
- For Research Use only
-
- Handling Advice
- Protect Components A and B from light and moisture.
- Storage
- -80 °C
- Storage Comment
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
-
- Target
- Factor Xa (FXa) (Activated Coagulation Factor X (FXa))
- Alternative Name
- Factor Xa (FXa Products)
- Synonyms
- FX Kit, FXA Kit, coagulation factor X Kit, F10 Kit
- Background
- Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits. FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca2+ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases.
-