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SensoLyte® Rh110 Enterokinase Assay Kit

FRET Fluorometric
Catalog No. ABIN1882457
  • Target See all TMPRSS15 Kits
    TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))
    Detection Method
    Fluorometric
    Application
    Fluorescence Resonance Energy Transfer Microscopy (FRET)
    Brand
    SensoLyte®
    Characteristics
    The SensoLyte® Rh110 Enterokinase Assay Kit employs a fluorogenic substrate for the detection of enterokinase activity. Upon protease cleavage, this substrate generates the Rh110 (rhodamine 110) fluorophore with bright green fluorescence that can be detected at excitation/emission=490/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. The assays are performed in a convenient 96-well microplate format.
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  • Comment

    FRET-based Assay Kit

    Restrictions
    For Research Use only
  • Handling Advice
    Protect Components A and B from light and from moisture.
    Storage
    -80 °C
    Storage Comment
    Store all kit components, except Component C, at -20 °C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience.
  • Target
    TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))
    Alternative Name
    Enterokinase (TMPRSS15 Products)
    Background
    Enterokinase or enteropeptidase is a heterodimeric serine protease produced by cells in the duodenal wall. It converts trypsinogen to trypsin, indirectly activating a number of pancreatic digestive enzymes. Enterokinase is a key enzyme for intestinal digestion of proteins. Its deficiency may cause severe protein malabsorption leading to impaired development and growth. Enterokinase also plays an important role in acute experimental and clinical pancreatitis. The high degree of specificity exhibited by enteropeptidase for cleaving affinity or other tags from recombinant proteins makes it an ideal tool for use in biochemical applications.
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