SensoLyte® 520 Cathepsin K Assay Kit
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- Target See all Cathepsin K (CTSK) Kits
- Cathepsin K (CTSK)
- Detection Method
- Fluorometric
- Application
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- Brand
- SensoLyte®
- Characteristics
- The SensoLyte® 520 Cathepsin K Activity Assay Kit uses a QXL™ 520/HiLyte Fluor™ 488 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin K enzyme in biological samples and purified enzyme preparations.
- Top Product
- Discover our top product CTSK ELISA Kit
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- Comment
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FRET-based Assay Kit
- Restrictions
- For Research Use only
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- Handling Advice
- Protect Components A and B from light and moisture.
- Storage
- -80 °C
- Storage Comment
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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- Target
- Cathepsin K (CTSK)
- Alternative Name
- Cathepsin K (CTSK Products)
- Synonyms
- CTS02 Kit, CTSO Kit, CTSO1 Kit, CTSO2 Kit, PKND Kit, PYCD Kit, AI323530 Kit, MMS10-Q Kit, Ms10q Kit, catK Kit, CTSK Kit, wu:fa95f03 Kit, wu:fb08b05 Kit, zgc:110367 Kit, cts02 Kit, ctso Kit, ctso1 Kit, ctso2 Kit, pknd Kit, pycd Kit, OC-2 Kit, catk Kit, cathepsin K Kit, cathepsin K L homeolog Kit, Cathepsin K Kit, CTSK Kit, Ctsk Kit, ctsk Kit, ctsk.L Kit, catk Kit
- Background
- Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.
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