SensoLyte® 520 Cathepsin D Assay Kit

Catalog No. ABIN1882439

Product Details

Target: Cathepsin D (CTSD)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL™ 520/HiLyte Fluor™ 488 labeled FRET peptide substrate for measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin D enzyme in biological samples and purified enzyme preparations.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light and moisture.

Storage: -80 °C

Storage Comment: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience

References

Feng, Tang, Kwon, Zong, Hawkins, Kitsis, Pessin: "High-fat diet-induced adipocyte cell death occurs through a cyclophilin D intrinsic signaling pathway independent of adipose tissue inflammation." in: Diabetes, Vol. 60, Issue 8, pp. 2134-43, (2011) (PubMed).

Li, Lu, Nguyen, Bae, Chapman, Feng, Hawkins, Pessin, Sears, Nguyen, Amidi, Watkins, Nguyen, Olefsky: "Functional heterogeneity of CD11c-positive adipose tissue macrophages in diet-induced obese mice." in: The Journal of biological chemistry, Vol. 285, Issue 20, pp. 15333-45, (2010) (PubMed).

Target Details

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D (CTSD Products)

Synonyms: catD Kit, fb93e11 Kit, fj17b09 Kit, wu:fb93e11 Kit, wu:fj17b09 Kit, LOC398557 Kit, Cathd Kit, CatD Kit, LOC100136761 Kit, CTSD Kit, DKFZp469J0315 Kit, DDBDRAFT_0205785 Kit, DDBDRAFT_0215012 Kit, DDB_0205785 Kit, DDB_0215012 Kit, CLN10 Kit, CPSD Kit, CD Kit, cathepsin D Kit, cathepsin D S homeolog Kit, lysosomal aspartic protease Kit, cathepsin D-like Kit, ctsd Kit, LOC398557 Kit, ctsd.S Kit, CatD Kit, LOC5567565 Kit, LOC100136761 Kit, CTSD Kit, Cathd Kit, ctsD Kit, catd Kit, Ctsd Kit, LOC443060 Kit

Background: Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breast cancer and Alzheimer disease.