SensoLyte® AFC Caspase Substrate Sampler Kit

Catalog No. ABIN1882426

Product Details

Target: Caspase (CASP)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® Caspase Substrate Kit contains a series of AFC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. It provides the best solution for identifying a suitable substrate for designing AFC-based caspase assays. All the components in the kit are available in bulk package.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Keep Components A and B away from light

Storage: -20 °C

Storage Comment: Store all components at -20 °C. For convenience, Components C and E can be stored at 4 °C.

References

Twiner, Hanagriff, Butler, Madhkoor, Doucette: "Induction of apoptosis pathways in several cell lines following exposure to the marine algal toxin azaspiracid." in: Chemical research in toxicology, Vol. 25, Issue 7, pp. 1493-501, (2012) (PubMed).

Gugliesi, De Andrea, Mondini, Cappello, Giovarelli, Shoenfeld, Meroni, Gariglio, Landolfo: "The proapoptotic activity of the Interferon-inducible gene IFI16 provides new insights into its etiopathogenetic role in autoimmunity." in: Journal of autoimmunity, Vol. 35, Issue 2, pp. 114-23, (2010) (PubMed).

Target Details

Target: Caspase (CASP)

Alternative Name: Caspase (CASP Products)

Synonyms: CG5370 Kit, DCP-1 Kit, DCP1 Kit, Dcp1 Kit, Dmel\\CG5370 Kit, ccp1 Kit, dcp-1 Kit, dcp1 Kit, l(2)01862 Kit, l(2)02132 Kit, Death caspase-1 Kit, Dcp-1 Kit

Background: Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=380 nm/500 nm). They are widely used to monitor caspase activity.