SensoLyte® ONPG b-Galactosidase Assay Kit

Catalog No. ABIN1882416

Product Details

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Detection Method: Colorimetric

Application: Enzyme Activity Assay (EAA)

Brand: SensoLyte®

Characteristics: The SensoLyte® ONPG beta-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl beta-D-galactopyranoside (ONPG) to determine the activity of beta-galactosidase in transfected cells or tissues. In the presence of beta-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to beta-galactosidase activity.

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Handling Advice: Keep component A away from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Component D can be stored at room temperature for convenience.

Target Details

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: beta-Galactosidase (GLB1 Products)

Synonyms: EBP Kit, ELNR1 Kit, MPS4B Kit, DER Kit, XR Kit, 0610038K04Rik Kit, 1810027P18Rik Kit, glb Kit, CG9092 Kit, Dmel\\CG9092 Kit, beta-GAL Kit, beta-Gal-1 Kit, beta-gal Kit, gal Kit, lacZ-1 Kit, AW125515 Kit, Bge Kit, Bgl Kit, Bgl-e Kit, Bgl-s Kit, Bgl-t Kit, Bgs Kit, Bgt Kit, C130097A14Rik Kit, BGAL Kit, zgc:110823 Kit, ECK0341 Kit, JW0335 Kit, GLB Kit, galactosidase beta 1 Kit, dicarbonyl and L-xylulose reductase Kit, dicarbonyl L-xylulose reductase Kit, beta-D-galactosidase Kit, beta-galactosidase Kit, beta galactosidase Kit, galactosidase, beta 1 Kit, beta-galactosidase-like Kit, GLB1 Kit, DCXR Kit, Dcxr Kit, lacZ Kit, SSP_RS00525 Kit, lacH Kit, ECL_03691 Kit, Gal Kit, Glb1 Kit, glb1 Kit, VV2_1327 Kit, LOC100056371 Kit

Target Type: Tag

Background: Beta-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells.